Job Description Summary The cell death/cellular bucket list pathways should be beneficial during infection but could be mutated and become detrimental in cancer. The Nozaki lab, stared at MUSC from Sep 1st , 2024, aims to uncover fundamental understanding of intestinal immunity during infection and inflammation, especially by focusing on cell death and its-associated pathways. The lab also aims to elucidate how intestinal inflammation affects the tumor evolution and its microenvironment, and how those mechanisms can be applied to therapeutics. To achieve this goal, my lab will develop novel mouse models as well as novel organoid models which can analyze the complicated death signaling and cell-to-cell, host-pathogen interactions. Under the direct supervision of the Principal Investigator, a Research Specialist I is needed to support immunology research for the analysis of mouse gut tissues and cells. Responsible for mouse maintenance, mouse sample preparation and analysis for PCR/qPCRs, Western Blots, flow cytometry, immunofluorescence, maintaining instrumentation and laboratory resources. Having experience of those techniques is a plus, but not necessarily required. The lab can provide various training opportunities. Entity Medical University of South Carolina (MUSC - Univ) Worker Type Employee Worker Sub-Type Research Grant Cost Center CC001032 COM PHARM Administration CC Pay Rate Type Hourly Pay Grade University-GEN06 Pay Range 37,944.00 - 49,266.00 - 60,690.000 Scheduled Weekly Hours 40 Work Shift Job Description 30% Performs preparation of mouse tissues for analysis. This includes mouse dissection / tissue harvesting, histopathology analysis, cell culture, molecular cloning, cell transfection / infection, quantitative real time PCR, Western blot, immunostaining, microscopy, and flow cytometry. 20% Performs mouse colony maintenance including mouse breeding, genotyping. 15% Maintains laboratory equipment by performing checklist systems suitability and required maintenance. 15% Assist in ordering reagents and laboratory supplies, and keeping the lab safe, clean and compliant by maintaining a neat work space, wearing appropriate personal protective equipment, and helping with labeling and inspection of laboratory items and solutions. 10% Maintain a detailed notebook of experiments performed and location of where data is stored. 10% Asist in preparing grant writing and IACUC protocols and amendments when appropriate. Preferred qualifications: A bachelor's degree in immunology, or molecular/cell biology. MUSC Minimum Training and Experience Requirements: A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience. Additional Job Description Minimum Requirements: A high school diploma and two years work experience in a clinical or research setting; a bachelor's degree in chemistry, biology, or other natural, life or health care science may be substituted for the two years of required experience. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then down 20 inches into centrifuge drum, unassisted. (Infrequent) Ability to lift 25 lbs. boxes, from floor level to maximum height of 72 inches, unassisted. (Infrequent) Ability to push/pull file boxes from one location to another at floor level. (Frequent) Ability to maneuver heavy equipment on bench tops, 50 lbs. or greater, unassisted. (Frequent) Ability to perform gross motor functions with frequent fine motor movements. (Continuous) Ability to maintain 20/40 vision, corrected, in one eye or with both eyes. (Continuous) Ability to match or discriminate between colors, normally. (Continuous) Ability to hear and recognize various alarms emitting from work area systems. (Continuous) Ability to hear and/or understand conversations. (Continuous) Ability to work in a darkroom for processing film, etc. (Continuous) Ability to be qualified physically for respirator use, initially and annually. (Continuous) Additional New Requirements: Ability to work rotating shifts as required. Ability to work overtime as required. Ability to learn and use new processes, tools and equipment as required. If you like working with energetic enthusiastic individuals, you will enjoy your career with us! The Medical University of South Carolina is an Equal Opportunity Employer. MUSC does not discriminate on the basis of race, color, religion or belief, age, sex, national origin, gender identity, sexual orientation, disability, protected veteran status, family or parental status, or any other status protected by state laws and/or federal regulations. All qualified applicants are encouraged to apply and will receive consideration for employment based upon applicable qualifications, merit and business need. Medical University of South Carolina participates in the federal E-Verify program to confirm the identity and employment authorization of all newly hired employees. For further information about the E-Verify program, please click here: http://www.uscis.gov/e-verify/employees Thank you for wanting to be part of the Medical University of South Carolina team. Whether you want to teach the next generation of health care leaders, innovate new business models, discover the next breakthrough or provide patient care, there's a role for you that fuels your passion and takes advantage of your skills. There are career opportunities available in academics, research, hospital medicine, physician practices and support services, from patient billing to IT. As an applicant, you can search jobs for all MUSC entities as well as search by category and location. MUSC attracts more than $250 million annually in research funding, making it the biggest magnet for biomedical, extramural research dollars of all institutions of higher learning in South Carolina. The Clinical and Translational Science Award (CTSA) Program aims to advance clinical and translational science to increase the speed at which new treatments become available to patients. MUSC Health, the clinical enterprise, operates a 750 bed medical center, which includes a nationally recognized Children’s Hospital, the Ashley River Tower (cardiovascular, digestive disease, and surgical oncology), Hollings Cancer Center (one of fewer than 70 elite National Cancer Institute designated centers), a Level I Trauma Center and the Institute of Psychiatry. In addition, there are more than 100 outreach clinics, hospital/health system affiliations, and telehealth sites. U.S. News & World Report placed us among the top one percent of all American hospitals, with 11 specialties in the top 50. The MUSC Medical Center is also one of only three Magnet® designated hospitals in South Carolina.

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